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KMID : 0894520080120030275
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Development & Reproduction
2008 Volume.12 No. 3 p.275 ~ p.281
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Effects of Bisphenol A and Nonylphenol on In Vitro Steroid Production in Matured Oocyte of Greenlings, Hexagrammos agrammus
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Hwang In-Joon
Kim Hyung-Bae
Baek Hea-Ja
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Abstract
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Endocrine disrupting chemiclas (EDCs) such as bisphenol A (BPA) and nonylphenol (NP) have estrogenic activity and can alter reproduction in fish. In the present study, the effects of BPA and NP on in vitro steroid production from oocytes of maturation stage (oocyte diameter≒1.88 ㎜) from the greenling (Hexagrammos agrammus) were evaluated. Oocytes were incubated with different concentrations of BPA and NP (0.1, 1, 10, 100 and 1,000 ng/㎖) in the presence or absence of 50 IU human chorionic gonadotropin (HCG) for 48 hours. After incubation, levels of 17α,20β-dihydroxy-4-pregnen-3-one (17α20βOHP), estradiol-17β (E₂) and testosterone (T) from incubated media were quantified by radioimmunoassay (RIA). In BPA treatment, 100 ng/㎖ of BPA stimulated E₂ production regardless HCG supplement. Every concentration of BPA inhibited T production without HCG although 0.1 ng/㎖ of BPA stimulated T production with HCG. In NP treatment, 10 ng/㎖ of NP stimulated 17α20βOHP and T production without HCG. 1 ng/㎖ of NP inhibited E₂ production. Taken toghther, these results suggest that BPA might have weak estrogen-agonistic effect and NP has estrogenantagonistic effect at final oocyte maturation stage of H. agrammus.
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KEYWORD
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Bisphenol A, Greenling, Nonylphenol, Oocyte maturation, Steroid, 17α, 20β-dihydroxy-4-pregnen-3-one, Estradiol-17β
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